Speaker:
Title:
Self-labeling Mtb Enables Identification and Enrichment of Mtb-derived Proteins from Infected Macrophages
Abstract:
The protein dynamics of both Mtb and host cells influence infection outcome. Previous studies of Mtb-infection have largely focused on host proteins due to the relative differences in biomass. To facilitate discrimination and enrichment of low-abundance Mtb proteins from host cells, we engineered Mtb that enzymatically label their own proteome. By enriching for labeled proteins, we were able to detect previously undetectable Mtb proteins in infected cells. This provides an unbiased way to identify Mtb proteins relevant in infection, potentially informing novel Mtb vaccine designs.