Seminars

Chromatin looping is essential for proper gene regulation, and its dysfunction is associated with cancer and other genetic diseases. Recent evidence suggests that chromatin loops are rare, fleeting structures, but this has not been studied on a genome-wide scale. We combine Micro-C and live imaging, two methods with complementary strengths, to estimate the absolute looping probabilities of 60,000+ loops across the mouse genome. We also analyzed the effects of different mechanisms and epigenomic factors on absolute looping probability.