Speaker:
Title:
Proximity Labeling as an Approach to Study the Phagosome Proteome
Abstract:
Professional phagocytes must internalize and digest extracellular material in a variety of contexts: from clearing apoptotic cells to killing pathogens. The phagosome formed in this process is a highly dynamic organelle which interacts with many cellular compartments to transition from an initially innocuous environment to one capable of these differing functions. Thus phagosomes are highly diverse, giving rise to different outcomes from similar initial conditions. We posit that this heterogeneity derives from the molecular composition of the phagosome, but holistic characterization of the phagosome composition has historically been complicated by its dynamism. Here, we adapt proximity labeling techniques to circumvent these complications and enable facile proteome-level analysis of the phagosomal lumen.