Speaker:
Title:
Stitch-seq: A Facile and High-throughput Perturbation Sequencing Method
Abstract:
Large-scale pooled CRISPR screens with high-complexity readouts, such as gene expression profiles, are a powerful approach to identify genes that affect cell behavior. However, the high cost of processing samples for single-cell transcriptome readout has hindered the scalability of gene expression screens. To address this, we present Stitch-seq, a low-cost genome-wide screening method with a targeted gene expression readout that does not need single-cell reagents or engineered reporter cell lines, making it generalizable to many biological systems.