Characterizing Bidirectional Promoter Transcriptional Dynamics Using Live-cell Nascent RNA Imaging
Approximately 11% of genes in the human genome are under the control of a bidirectional promoter (BDP), defined as two divergent genes with <1kb between their transcription start sites. Despite an enrichment for essential and oncogenic genes, the regulatory function of BDPs remains largely unknown. Here we aim to study BDP transcriptional dynamics using dual-color live-cell nascent RNA imaging of the endogenous NIPBL/AS1 bidirectional promoter. This system will provide a basis for in-depth, mechanistic study of BDP transcriptional regulation.