Speaker:
Title:
Pooled Genetic Screens to Interrogate TCR Signaling Networks
Abstract:
The protein Linker for Activation of T cells (LAT) is a membrane-associated adapter required for signal amplification and diversification from the T cell receptor. The discovery of inducible protein interactions and higher-order assemblies mediated by LAT post-translational modifications and binding surfaces suggests that LAT is a platform regulating downstream pathways. To achieve a comprehensive picture of LAT molecular function, we demonstrate a high-content screen in which a series of mutants in the LAT protein are analyzed to characterize their effect on T cell activation.