Speaker:
Title:
Physical Expansion of a Nematode Enables Whole-organism In Situ Analysis With Nanoscale Resolution
Abstract:
Studies of biological systems will benefit from a robust method to collect molecular information over an entire organism at high spatial resolution. We have developed a method to physically expand fixed tissues of the nematode model C. elegans with high isotropy, while allowing simultaneous detection of fluorescent proteins, DNAs and RNAs at sub-cellular resolution. We demonstrate the utility of this method for analyzing neuronal connectivity, transcriptomic profiles and other structural features of the entire animal.