Speaker:
Title:
Quantitative Time Lapse Analysis of Intracolony Heterogeneity in Mesenchymal Stromal Cell Cultures
Abstract:
The emergence of heterogeneity in mesenchymal stromal cell (MSC) populations during in vitro expansion holds direct implications for basic research and therapeutic applications. The potential for heterogeneity even within single-cell-derived stem cell
colonies suggests that impactful progress requires increased understanding of the conditions that cause and can mitigate functional heterogeneity in MSC cultures. Toward this aim, we report our first steps in elucidating the causes of heterogeneity onset in MSCs using time-lapse image analysis.