BATS

Date/Time:

May 12, 2017 - 12:00 PM

Location:

Hosts:

Bevin P. Engelward

Speaker:

Title:

Quantitative Time Lapse Analysis of Intracolony Heterogeneity in Mesenchymal Stromal Cell Cultures

Abstract:

The emergence of heterogeneity in mesenchymal stromal cell (MSC) populations during in vitro expansion holds direct implications for basic research and therapeutic applications. The potential for heterogeneity even within single-cell-derived stem cell colonies suggests that impactful progress requires increased understanding of the conditions that cause and can mitigate functional heterogeneity in MSC cultures. Toward this aim, we report our first steps in elucidating the causes of heterogeneity onset in MSCs using time-lapse image analysis.

Speaker:

Title:

Cellular Self-reporting

Abstract:

RNAseq is an important analytical technique for the study of mammalian cells — its ability to obtain highly resolved molecular phenotypes from individual cells is transforming the way we define cell states, as well as understand cell circuitry in the context of disease and development. However, the causal relationships behind biological dynamics are lost in static RNAseq snapshots. This talk will outline a technology to allow cells to self-report their internal states in time-lapse measurements by exporting a portion of their mRNA.