Speaker:
Title:
Enhancing the CRISPR-Cas Genome Engineering Toolbox
Abstract:
Targeted genome editing using microbial CRISPR-Cas systems has
enabled a broad range of applications in research and medicine. However,
naturally-occurring Cas enzymes that have been harnessed for use in
eukaryotic cells have a number of significant drawbacks, including the
potential for off-target DNA mutagenesis or a restricted targeting range.
This talk explores some recent developments in the use of structure-guided
and directed evolution-based protein engineering of Cas enzymes as a novel
method to address these concerns.