BATS

Date/Time:

Apr 1, 2016 - 12:00 PM

Location:

Hosts:

Bevin P. Engelward

Speaker:

Title:

A Yeast Display/Secretion System for the Rapid Generation of Renewable Recombinant Abs

Abstract:

Many commercial antibodies fail to perform as desired in laboratory assays, and the process of generating new antibodies can be a long, risk-filled process. Here we present a yeast display based system to facilitate the rapid isolation of validated antibody-like constructs in as little as one month. This system is able to both display antibody on the surface of yeast, suitable for binder isolation and characterization, as well as secrete antibody, allowing for cloning-free expression of promising candidates.

Speaker:

Title:

Heterogeneous Molecular Dynamics Revealed Through Live, Single-Cell Imaging

Abstract:

The study of the dynamic motion of single molecules or ensembles of molecules within cells can lend information as to those molecules’ activity or function. Here, we investigate the cell wall synthetic process of Bacillus subtilis in order to elucidate the mechanisms behind construction of the primary encoder of cell shape in this organism. Using live-cell, single-molecule imaging, a technique we employ alongside small-molecule antibiotic perturbations, we probe molecular motion of the enzymes and filaments involved in cell wall construction.