Speaker:
Title:
Characterizing Bidirectional Promoter Transcriptional Dynamics Using Live-cell Nascent RNA Imaging
Abstract:
Approximately 11% of human genes are under the control of a bidirectional promoter (BDP), defined as two divergent genes with <1kb between their transcription start sites. Despite an enrichment for essential and oncogenic genes, the regulatory function of BDPs remains largely unknown. Here we aim to study BDP transcriptional dynamics using two-color live-cell nascent RNA imaging of 12+ human bidirectional promoters. This will provide insight into broad BDP dynamics and serve as the basis for in-depth mechanistic study of BDP regulation.