Applying Proximity Labeling to Study the Pathogen-containing Phagosome
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, is a leading cause of death and disease worldwide. Mtb survives and replicates inside of the macrophage phagosome, as Mtb has evolved to arrest phagosomal maturation and impair typical macrophage killing mechanisms. To better understand how Mtb manipulates the phagosome, we need a technique which enables unbiased labeling and purification of pathogen-interacting phagosomal components. Here, we apply proximity labeling to the pathogen-containing phagosome, enabling comparative studies of pathogen manipulation of the phagosome.