Stitch-seq: A Facile and High-throughput Perturbation Sequencing Method
Genetic perturbation screens, such as CRISPR screens, are invaluable tools for elucidating the relationships between genes and phenotypes. Recent technological advances have enabled the profiling of each cell’s complete transcriptional state following a perturbation. However, this is accomplished using single cell RNA sequencing, which is both expensive and laborious, making it impractical for genome-wide screens. Here, we present a facile and inexpensive perturbation sequencing method that can interrogate tens of thousands of genetic elements at once by selectively profiling key aspects of the cell state.