Engineering DNA Origami Nanoparticles as a Modular Vaccine Platform to Investigate Humoral Immunity
Wireframe DNA origami can be leveraged as a structural scaffold to organize proteins and other bioactive molecules at the nanoscale. The highly programmable nature of nucleic acids provides precise control over the geometry and stoichiometry of protein organization, which is difficult to achieve using classical protein nanotechnology. This level of control is particularly valuable for investigating nanoscale mechanisms of immune cell activation. Here we demonstrate the utility of DNA origami nanoparticles displaying engineered immunogens as a vaccine platform in vivo, which we propose to use to elucidate nanoscale design rules for subunit vaccines.