Jacqueline Gerritsen (WHITE LAB)

Mechanistic Characterization of the Epidermal Growth Factor Receptor Signaling Network

Dysregulated in many cancers, the epidermal growth factor receptor (egfr) heavily relies on tyrosine phosphorylation for signal transduction. Despite widespread efforts in targeting egfr, results in the clinic have been mixed due to a lack of understanding of the individual roles of its tyrosine residues. Here, we present a phosphoproteomic approach to study the signaling network using a site-specific model. Our dataset highlights the resilience of this system as well as components of the network we may leverage in future targeting strategies.

Jonathan Hsu (COLLINS, JOUNG, & PINELLO LABS)

PrimeDesign Software for Rapid and Simplified Design of Prime Editing Guide RNAs

Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens.