BATS

Date/Time:

Oct 23, 2020 - 12:00 PM

Location:

Hosts:

Bevin Engelward

Speaker:

Title:

Design and Characterization of DNA Origami for Immunostimulation

Abstract:

Many cancer immunotherapy strategies have been focused on manipulating and stimulating the adaptive immune response against tumors, whereas the potential impact of the innate immune system has yet to be thoroughly explored. Here we discuss the development of rationally designed DNA nanostructures for programmable stimulation of the innate immune response.

Speaker:

Title:

Isolating Signal Peptide-mediated Selection Bias in Yeast Display

Abstract:

Yeast surface display has been adapted as a technique to screen millions of protein variants simultaneously. While often successful, a number of factors can complicate using this technique to systemic scan for binders, potentially leading to false negatives and systematic absences in datasets. Here, we determine the interplay between variant sequence and signal peptide cleavage and discuss early efforts to empirically understand these emergent biases.