Methods to Isolate Tumor-Specific Antibodies Induced by Immunotherapy
Combination immunotherapy has been shown to potently eliminate tumors and induce immunological memory in mouse models. Mice that are cured by this therapy develop endogenous antibodies that are protective against the tumor. Interestingly, endogenous antibodies found in a subset of these mice show specificity toward other tumor cells, suggesting the possible existence of shared antigens. To better understand the specificity of these antibodies, this work explores different approaches to isolating tumor-specific antibodies from mouse organs.