Dynamic Metabolic Engineering in Bacteria Using Quorum Sensing Circuits
Traditional methods to engineer strains for bioproduction of desired compounds have involved overexpressing select genes or deleting competing pathways. In more complex scenarios, such as when the competing pathway is essential to cell survival, these “static” strategies cannot be implemented. Using quorum sensing parts, we have engineered circuits to dynamically modulate expression of any target gene. These circuits allow strains to autonomously downregulate competing pathways that cannot be knocked out, and siphon flux to product formation.