Speaker:
Title:
Understanding T Cell Diversity in Peanut Allergy
Abstract:
Peanut allergy is a type 1 hypersensitivity condition driven
by a combination of T cells and B cells. Oral immunotherapy (OIT) is a
promising treatment for peanut allergy, but long-term clinical outcomes have
been variable, and the cellular mechanism of the therapy is still debated.
Here, we propose using T cell receptor sequencing to characterize the
dynamics of T cell repertoires in patients undergoing peanut OIT. In
particular, we seek to understand the importance of T cell clonal responses
in peanut OIT.
Speaker:
Title:
Nanoscale, Multiplexed, Biomolecular Mapping in Brain Circuits
Abstract:
Nanoscale imaging throughout brain circuits in a
multiplexed fashion would enable the molecular characterization of cell
types, synapses, and signaling pathways. Although many multiplexing
strategies exist, it remains difficult to use such technologies in large 3-D
tissues. Recently we developed an approach to physically magnify tissues,
which we call Expansion Microscopy (ExM). ExM isotropically magnifies
tissues, enabling super-resolution imaging on fast diffraction-limited
microscopes. Here we present methods using this principle that enable the
multiplexed, and nanoscale imaging of transcripts in brain tissue sections.