Seminars

Plasmodium falciparum is the deadliest cause of human malaria. Although front-line antimalarials like
artemisinin (ART) have significantly reduced the malaria burden, cases of P. falciparum parasites that survive ART exposure are increasing. Dysregulation of the Pfkelch13 gene is implicated in ART resistance, though its precise function within the parasite remains unclear. We utilized a conditional knockdown (cKD) system in an assay designed to modulate Pfkelch13 expression and assess the effect on P. falciparum survival and resistance to ART.