Seminars

Radiation-tolerant filamentous fungi can be engineered for in situ biomanufacturing to support long-duration space missions. Engineering these organisms requires single-copy DNA integration in accessible
chromosomal regions and mycelial characterization. We combine Agrobacterium delivery with site-specific recombinases for targeted integration, eliminating transcriptional bias. Large-particle flow cytometry resolves circuit performance and mycelium-to-mycelium heterogeneity. This enables distributed biomanufacturing under austere conditions.

T cell receptors (TCRs) bind mutant peptides presented on the major histocompatibility complex (pMHC), enabling disease detection and elimination. However, the diversity and complexity of TCR:pMHC interactions makes screening functional TCR:pMHC pairs a challenge at scale. To address this, we developed a rapid, high-throughput assay which can detect and sequence activating TCR:pMHC pairs in their native cellular context. We aim to apply this platform to generate large TCR:pMHC datasets, which can inform TCR-T cell therapy development.