Engineering Endogenous Virus-like Proteins for mRNA Delivery
Delivery of gene therapy to target tissues remains an unsolved challenge. Current delivery vehicles consist primarily of viruses and lipid nanoparticles, which have drawbacks including vector immunogenicity. To overcome this we engineered virus-like proteins expressed in the human body to deliver mRNA to target cells. We found the retrotransposon-derived gene PEG10 packages its own mRNA and can be reprogrammed to deliver an mRNA of interest to target cells. We hope this platform will allow for modular, non-immunogenic delivery of gene therapy.