Engineering a Mammalian Toggle Switch that Functions over Long Time Scales
Cell therapies have yielded breakthrough cures in cancer and genetic diseases. Synthetic biological circuits may enable sophisticated cell therapies that respond to patient biomarkers and inputs from clinicians. However, epigenetic silencing causes traditional mammalian genetic circuits to lose function within a few weeks, impeding applications in cell therapies. Here, I will discuss the development of a mammalian toggle switch that remains functional over long time scales.