Current malaria vaccines utilize either subunit or whole-cell approaches and are inefficient in offering protection. I propose a combined approach wherein a whole parasite serves as a chassis for protein presentation to reap the benefits of specific, chosen antigens on a non-specified protein-presenting surface of the parasite membrane. Parasite lines were generated with conditional expression of protein fusions for a vaccine prototype. This should protect across the parasite life cycle to combat infection, growth, and transmission.

CAR T cell therapies have produced unprecedented responses in treating B cell cancers, but they are often accompanied by severe side effects and have not translated to many other disease indications. Several groups have shown that modifying the signaling components of these chimeric antigen receptors can introduce novel and useful T cell functions. Here, we propose to expedite this discovery process by creating a CAR library with diverse signaling domains and selecting for CARs that produce clinically relevant functional phenotypes.