Before beginning laboratory research, all personnel must do the following:
- Read the BE Chemical Hygiene Plan
- Receive Chemical Hygiene training by completing one of the following options video, web or classroom training;
- Receive Managing Hazardous Waste training by completing one of the following options web or classroom training;
- Review the quiz questions (see the appendix of BE CHP) with their principle investigator, or other approved personnel, who will co-sign a sheet confirming that these training requirements have been met.
- Submit both the BE Chemical Hygiene Clearance Form and the Web-Based Training "Certificate of Completion" to BE Headquarters.
Principal Investigators are responsible for determining which agents used by members of their group are particularly hazardous, for posting a list of these agents, and for approving SOPs. Particularly hazardous substances include reproductive toxins, substances that have and oral LD50 of less than 50 mg/kg, a skin contact LD50 of less than 200 mg/kg, an inhalation LC50 of less than 200 ppm/air, and select carcinogens (as defined by the OSHA health standards 29 CFR part 1910; see Appendix M or this page for links to lists of known and suspected carcinogens). SOPs must include a description of the health hazard, flammability, reactivity, chemical incompatibilities, appropriate precautions, proper disposal, and action to be taken in case of exposure or a spill.
All personnel under the BE Chemical Hygiene Plan must practice safe laboratory practices as described below. Failure to comply these regulations may lead to dismissal.
- No food, beverage, tobacco product, or medicine is to be inhaled, ingested, or injected in any laboratory. No food, beverage, tobacco product or medical wrappers or containers may be carried into or discarded in any laboratory.
- Work only with materials when you know their flammability, reactivity, toxicity, safe handling, disposal, and emergency procedures.
- You must have a copy of an approved Standard Operating Procedure (SOP) prior to use of particularly hazardous substances. Principal Investigators are responsible for determining which agents used by members of their group are particularly hazardous, for posting a list of these agents, and for approving SOPs. Particularly hazardous substances include reproductive toxins, substances that have and oral LD50 of less than 50 mg/kg, a skin contact LD50 of less than 200 mg/kg, an inhalation LC50 of less than 200 ppm/air, and select carcinogens (as defined by the OSHA health standards 29 CFR part 1910; see Appendix M, page 95). SOPs must include a description of the health hazard, flammability, reactivity, chemical incompatibilities, appropriate precautions, proper disposal, and action to be taken in case of exposure or a spill.
- Substances designated as "particularly hazardous" must be labeled with a red dot sticker. Toxicity warning signs or symbols must be prominently visible on containers of particularly hazardous substances.
- Laboratory coats and gloves must be worn when handling hazardous chemicals.
- Always remove gloves (even if you think they are clean!) before touching computer keyboards, computer mice, door handles and telephones. If it is necessary to keep a glove on to transport a hazardous material in the hallway, remove a glove from one hand in order to open doors or use a clean paper towel.
- All procedures involving volatile materials or aerosols of a toxic or flammable nature must be performed in an exhausting hood.
- To minimize the risk of eye injury, it is recommended that eye protection be worn on a regular basis. Certified safety glasses with side shields or goggles are absolutely required in the following situations:
- When handling corrosive, particularly hazardous, or radioactive substances that could cause eye injury;
- When performing chemical operations that could explode or implode;
- When in a room where there is a reasonable danger of eye injury from flying particles/objects. Due to risk of flying objects, certified safety glasses are absolutely required in the following rooms: 56-638 (west half), 654 (east half), 663, 686, 670, 691, 16-775. Access to safety glasses must be available outside these rooms for use by visitors.
- Never pipette by mouth.
- Hazardous materials in breakable containers must be contained within a shatter-proof secondary container during transportation (e.g., when carrying ethanol down a hallway).
- All hazardous waste containers require a red tag and all contents must be written out in English (no chemical abbreviations; red tags are available from the Safety Office, 3-4637). Waste containers must be stored in satellite accumulation areas. There can be no more than one container with the same type of waste in one satellite accumulation area. Lids to hazardous waste containers must always be kept closed, unless in use (one cannot leave an area with an open hazardous waste container).
- All sharp materials (e.g., pipette tips, needles, plastic pipettes, glass pipettes, plastic 15 ml tubes, broken glass, and razor blades - anything that could puncture a garbage bag) must be disposed of in a sharps box or a red plastic sharps container. These must be autoclaved prior to disposal if they are contaminated with biohazardous materials. Sharps contaminated with hazardous materials must be collected in puncture-proof containers labeled with red tags.
- Perform a safety check after each experiment. Make sure gas, water, flames, vacuum, and hot plates are turned off. Decontaminate your work area after using biohazards.
- Wash hands before leaving the laboratory.
- Exits and passageways must be kept clear at all times. Know the locations of fire extinguishers, emergency wash facilities, fire alarm pull stations, telephones and emergency exits.