BATS

Date/Time:

Apr 29, 2022 - 12:00 PM

Location:

Hosts:

Bevin Engelward

Speaker:

Title:

Elucidating the Surface Proteome of Mycobacterium tuberculosis

Abstract:

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is a leading cause of infectious disease death. Current vaccine candidates fail to protect adults from pulmonary TB. Determining which antigen(s) to include in vaccines is a challenge magnified by a partial picture of host accessible proteins. Here, we adapt a protein shaving protocol to identify proteins from the Mtb surface proteome and characterize protein hits to better inform our understanding of Mtb surfaceome composition and expand the pool of potential vaccine antigens.

Speaker:

Title:

Uncovering the OCT4 Expression Trajectory of Successful Reprogramming in Human Fibroblasts

Abstract:

Reprogramming of HDFs to pluripotency via the overexpression of key transcription factors (TFs) is inefficient and produces immature colonies. This may stem from a lack of understanding of the optimal TF dynamics required to drive this process. To chart the OCT4 trajectories in those few cells that yield iPSCs, we develop a genetic controller that sets a wide range of total OCT4 without interference from endogenous expression, coupled with live imaging to track OCT4 during reprogramming. Our data reveal that distinct OCT4 trajectories generate iPSCs of varying quality. Our approach can be exploited in any cell fate programming application.