BATS

Date/Time:

May 28, 2021 - 12:00 PM

Location:

Hosts:

Bevin Engelward

Speaker:

Title:

Compact RNA Editors Using Small Cas13 Proteins

Abstract:

CRISPR-Cas13 systems have been developed for precise RNA editing, an attractive therapeutic strategy when temporary changes are desirable or when DNA editing is not possible. We identified and characterized an ultra-small family of Cas13b, Cas13bt, which we show mediates mammalian transcript knockdown. By functionalizing Cas13bt with adenosine and cytosine deaminase domains, we engineered compact variants of REPAIR and RESCUE RNA editors and demonstrated functional packaging in a single AAV vector, which may be amenable for in vivo use.

Speaker:

Title:

Abstract: