Investigation of MAPK Inhibition Using Analog-sensitive Kinase
RAS oncoprotein is a major driver of many different cancer types. However, we currently do not have effective therapeutics to counter it, which shifts our attention to mitogen-activated protein kinase (MAPK), which is one of the primary effectors of RAS signaling. MAPK1, more famously known as ERK2, phosphorylates numerous substrates and identifying direct ERK2 substrates become more challenging with additional phosphorylation by different kinases. Here, we propose an approach to identify direct ERK2 substrates using analog-sensitive kinase.