Elucidating Molecular Interactions in Synaptogenesis with Optical Pooled Screens
Synaptogenesis, the formation of new neuronal connections, plays a critical role in shaping the connectivity and function of the nervous system. Understanding the diverse molecular mechanisms driving synapse formation has remained an outstanding goal in neurobiology. However, current methods for studying synaptogenic regulators lack the throughput required to systematically probe their interactions. Here, I will discuss our efforts to develop an image-based pooled screening approach to map the regulatory networks underlying synaptogenesis.