Real-time, Programmable Detection of Nucleic Acid Sequences in Living Cells
Nucleic acid sequence can provide information about cell state and biological activity in the environment. Existing detection methods are incompatible with living cells. We propose to detect intracellular nucleic acid sequences by two orthogonal dCas13s that bind adjacent RNA sites and thus induce an intein-mediated release of a sigma factor. Integration of this sensor with a DNA uptake pathway native to Bacillus subtilis could enable detection of environmental DNA for purposes such as pathogen or resistancegene monitoring.