Speaker:
Title:
An Unbiased Determination of Class II MHC Peptide Repertoires via Yeast-displayed Libraries
Abstract:
CD4+ ‘helper’ T cell function relies upon T cell receptor recognition of peptides displayed by class II MHCs. Algorithms developed to predict which peptides can bind to class II MHCs – and therefore be recognized by CD4+ T cells – suffer from significant inaccuracies due to a lack of diverse high-quality peptide data. Here, we present a yeast-displayed platform to screen large unbiased peptide libraries for binding to multiple class II alleles, discovering peptide binding motifs that can significantly improve current prediction algorithms.