BATS

Date/Time:

Dec 2, 2016 - 12:00 PM

Location:

Hosts:

Bevin P. Engelward

Speaker:

Title:

Synthetic Hydrogels for Studying Morphogenesis in Engineered Multicellular Systems

Abstract:

Significant progress has been made in the development of microphysiological model systems designed to mimic human tissue; however, many of these models still do not sufficiently recapitulate the structure and function of the in vivo environment. In this work, I employ synthetic, engineerable hydrogels to examine the fundamental interactions governing tissue morphogenesis in vitro, with the long-term goal of advancing our ability to design physiologically relevant model systems.

Speaker:

Title:

A Path to Genome-wide Chromatin Conformation Imaging

Abstract:

The proposed thesis seeks to enable and demonstrate in situ imaging of chromatin conformation in individual genomes in cells and tissues. In situ hybridization probes designed using the Oligopaints method will be applied to genomic targets followed by physical magnification using expansion microscopy (ExM), enabling super-resolution imaging of genomic loci at a resolution of ~ 20nm. Kilobase-scale sequence resolution will be obtained by sequencing individual ISH probes in situ using fluorescent in situ sequencing (FISSEQ).