Development of Tools for the Facilitated Engineering of the Bacterial Genome
In bacteria, genetic systems are engineered in plasmids due to the ease of use and the high efficiency. However, these systems burden the cell and require antibiotics to maintain them. While genome integration resolves such issues, the process is not straightforward. Here, we introduce a transposon Tn5-based system that integrates genetic systems to genomic locations with the specified expression levels, by using a one-step transformation. With this tool, we will introduce synthetic systems to the genome of a probiotic strain E. coli Nissle.