BATS

Date/Time: 

Nov 22, 2019 - 12:00 PM EST

Title: 

Identification of DNA Single-strand Breaks with Single Nucleotide Resolution

Abstract: 

Defective repair of DNA single-strand breaks (SSBs) is implicated in neurodegenerative disorders and cancer. Furthermore, recent advances in genome editing rely on nicking DNA at specific loci and may be susceptible to generating off-target SSBs. Despite this, current methods for measuring such breaks are lacking. Here, we present NickSeq, a method that is uniquely capable of identifying SSBs with strand-specific single nucleotide resolution in an unbiased manner. We then utilize this method to identify the off-target activity of Cas9 nickase.

Title: 

Metagenomic Mining and Characterization of Novel CRISPR Systems

Abstract: 

Defective repair of DNA single-strand breaks (SSBs) is implicated in neurodegenerative disorders and cancer. Furthermore, recent advances in genome editing rely on nicking DNA at specific loci and may be susceptible to generating off-target SSBs. Despite this, current methods for measuring such breaks are lacking. Here, we present NickSeq, a method that is uniquely capable of identifying SSBs with strand-specific single nucleotide resolution in an unbiased manner. We then utilize this method to identify the off-target activity of Cas9 nickase.